SARS?CoV?2 nucleocapsid protein intranasal inoculation induces local and systemic T cell responses in mice

SARS-CoV-2 nucleocapsid (N) protein has been proposed as a good vaccine target. N-specific T cells were observed in N immunized mice and COVID-19 convalescents. It is of importance to identify the cell responses triggered by protein. Intradermal immunization with SARS-CoV was demonstrated elicit non-protective which may be avoided intranasal vaccination. Therefore, we conducted vaccination BALB/c recombinant adenovirus type-5 expressing Such procedure induced CD8 lung. Meanwhile CD4 spleen, associated robust antibody production. Our study further supports notion that can work target for development.